Afuco™ Anti-Human LRRC15 ADCC Recombinant Antibody, ADCC Enhanced

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Reliable in Oncology Research

This antibody demonstrates exceptional reliability in oncology studies, providing consistent and reproducible results. Its enhanced ADCC activity ensures accurate identification of LRRC15.

Effective in Immunotherapy Studies

In immunotherapy research, the anti-human LRRC15 ADCC recombinant antibody is highly effective. It enhances antibody-dependent cellular cytotoxicity, making it a crucial tool for developing targeted cancer therapies.

High Specificity

Creative Biolabs' anti-human LRRC15 ADCC recombinant antibody exhibits high specificity in various assays. This specificity ensures accurate results, essential for studying the role of LRRC15 in tumor biology.

Q&As

1. What is the recommended procedure for preparing anti-human LRRC15 ADCC recombinant antibody for an ELISA assay?

   A: To prepare the anti-human LRRC15 ADCC recombinant antibody for ELISA, dilute the antibody to the recommended concentration using the ELISA coating buffer. Ensure all reagents are at room temperature, coat the wells with the antibody, and block non-specific binding sites with a blocking buffer. Follow the specific ELISA kit instructions for further steps.

2. How can one optimize the concentration of anti-human LRRC15 ADCC recombinant antibody for use in immunohistochemistry (IHC)?

   A: To optimize the concentration for IHC, perform a titration experiment starting from a broad range, such as 1:50 to 1:200. Apply different dilutions to tissue sections, incubate, and visualize staining intensity. The optimal dilution provides strong specific staining with minimal background.

3. What is the protocol for using anti-human LRRC15 ADCC recombinant antibody in Western blot analysis?

   A: For Western blot, load the protein samples onto an SDS-PAGE gel and transfer them to a PVDF or nitrocellulose membrane. Incubate the membrane with the antibody diluted in blocking buffer (e.g., 1:1000 to 1:10000). Wash and incubate with an HRP-conjugated secondary antibody, followed by detection using a chemiluminescent substrate.

4. Can anti-human LRRC15 ADCC recombinant antibody be used for immunoprecipitation? If so, what is the protocol?

   A: Yes, it can be used for immunoprecipitation. Incubate the antibody with cell lysates to form an antibody-antigen complex. Add protein A/G agarose beads to capture the complex. Wash the beads thoroughly to remove non-specific proteins and elute the bound proteins for further analysis.

5. What is the best method for optimizing anti-human LRRC15 ADCC recombinant antibody concentration in flow cytometry?

   A: Perform a titration experiment by staining cells with a range of antibody concentrations. Analyze the samples using flow cytometry to determine the optimal concentration that provides clear separation between positive and negative populations with minimal background staining.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# AFC-480CL, RRID: AB_3111279)

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

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